RSPO2和β-catenin在羊驼不同部位皮肤中表达差异的研究

doi:10.11843/j.issn.0366-6964.2016.05.009

Abstract

Abstract:

Alpaca fiber is the ideal material for the textile industry.Its length，fineness and curvature are different in the back，leg and ear.In order to investigate the possible relationship between RSPO2 and β-catenin with the characteristics of alpaca fleece，the expressions of RSPO2 and β-catenin in back，leg and ear skins of alpaca were analyzed using immunohistochemistry，real-time PCR and Western blotting.The immunohistochemistry analysis demonstrated that the positive reaction of RSPO2 was localized in the cytoplasmic of dermal papilla cells，hair follicle matrix，inner root sheath，outer root sheath，sweat glands and epidermis cells，in particular，the inner root sheath showed strongest positive reaction.The positive reaction of β-catenin was localized in hair follicle matrix，inner root sheath，outer root sheath，sweat glands and epidermis cells.Real-time PCR showed that the expression of RSPO2 in back and leg skins were 7.633 folds（P＜0.05） and 5.602 folds（P＜0.05），respectively，in comparison with that in ear skins.The expression of β-catenin in back and leg skins were 3.689 folds（P＜0.05） and 2.067 folds（P＞0.05），respectively，in comparison with that in ear skins.Western blot analysis showed that the positive band of RSPO2(26 ku) and β-catenin (86 ku) were strongest in back skins and weakest in ear skins with significant difference (P＜0.05）.The positive band of RSPO2 in leg skins was significantly stronger than that in ear skins（P＜0.05）.The results suggested that RSPO2 and β-catenin were positively associated with regulating the length，curvature and fineness formation of alpaca fleece.

CLC Number:

S829.9

GAO Shu-yuan，YU Xiu-ju，CHENG Jia-qi，SONG Ya-jun，ZHAO Bing-ling，NIU Shu，CAO Xiao-rui，HE Xiao-yan. Different Expression of RSPO2 and β-catenin in the Different Sites of Alpaca Skins[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(5): 931-937.

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Different Expression of RSPO2 and β-catenin in the Different Sites of Alpaca Skins

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